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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has become noticed in several tumors, which leads to chromosome instability, and so may lead to tumorigenesis. This protein was observed to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a crucial part in marketing mobile-cycle development during the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, happen to be described. Two added splice variants had been claimed but thorough nucleotide sequence info is not really but available.
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Blocking peptides are peptides that bind specifically to the focus on antibody and block antibody binding. These peptide usually contains the epitope identified with the antibody.
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This guidebook outlines even more possible optimization of cellular and immunoassay parameters to ensure the absolute best benefits are...
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This guide outlines additional achievable optimization of cellular and immunoassay parameters to make certain the absolute best final results are...
Antibodies certain to the blocking peptide now not bind on the epitope over the target protein. By evaluating the staining with the blocked antibody as opposed to the antibody by yourself, one can see which staining is particular.
Target Information Cyclin E belongs to the really conserved cyclin spouse and children, whose users are characterized by a remarkable periodicity in protein abundance with the mobile cycle. Cyclins function as regulators of CDK kinases. Distinctive cyclins show distinct expression and degradation styles which add for the temporal coordination of each mitotic party. Cyclin E varieties a complex with and capabilities being a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S transition. This protein accumulates for the G1-S phase boundary and is particularly degraded as cells progress via S section.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by each antibodies...
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